SDS-PAGE
SDS-PAGE for Anti-Human ADAMTS13 Antibody .FC
Flow-cytometry using anti-human ADAMTS13 antibody. ADAMTS13 Transfected CHO cells were stained with an irrelevant antibody or an anti-human ADAMTS13 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human ADAMTS13 antibody. HepG2 cells were stained with an irrelevant antibody or an anti-human ADAMTS13 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC- conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using PE anti-human ADAMTS13 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human ADAMTS13 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human ADAMTS13 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,lambda |
| Clone Number | J4Z9 |
| Host Species | Mouse |
| Tested Applications | BlockingELISAFCMSPR |
| ELISA:1:2000-1:20000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, 3.4.24.87, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease, ADAMTS13, C9orf8, UNQ6102/PRO20085 |
|---|---|
| Function | Anti-Human ADAMTS13 Antibody (3H9)[3H9] (HV675013) is amouse monoclonalantibody detectingA disintegrin and metalloproteinase with thrombospondin m...inBlocking, ELISA, FCM, SPR. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | Q76LX8 |
|---|
SDS-PAGE
SDS-PAGE for Anti-Human ADAMTS13 Antibody .
FC
Flow-cytometry using anti-human ADAMTS13 antibody. ADAMTS13 Transfected CHO cells were stained with an irrelevant antibody or an anti-human ADAMTS13 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human ADAMTS13 antibody. HepG2 cells were stained with an irrelevant antibody or an anti-human ADAMTS13 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC- conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using PE anti-human ADAMTS13 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody or an PE anti-human ADAMTS13 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
