FC
Flow-cytometry using anti-human IL4 antibody.IL4 Transfected CHO cells were stained with an irrelevant antibody or an anti-human IL4 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.SDS-PAGE
SDS-PAGE for Anti-Human IL4 Antibody .| Product Name | Anti-Human IL4 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1,k |
| Clone Number | V1X9 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMIHCIPNeutralization |
| ELISA:1:2000-1:20000 IHC:1:100-1:1000 IP:1:10-1:100 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Binetrakin, B-cell stimulatory factor 1, Pitrakinra, Lymphocyte stimulatory factor 1, IL-4, BSF-1, IL4, Interleukin-4 |
|---|---|
| Function | Anti-Human IL4 Antibody (8D4-8)[8D4-8] (HY457013) is amouse monoclonalantibody detectingBinetrakininELISA, FCM, IHC, IP, Neutralization. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P05112 |
|---|
FC
Flow-cytometry using anti-human IL4 antibody.IL4 Transfected CHO cells were stained with an irrelevant antibody or an anti-human IL4 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human IL4 Antibody .| Application Notes | ELISA:1:2000-1:20000 IHC:1:100-1:1000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
