Bioactivity
Detects MMP14 in indirect ELISAs.SDS-PAGE
SDS-PAGE for Anti-Human MMP14 Antibody .FC
Flow-cytometry using anti-human MMP14 antibody.MDA-MB-231 cells were stained with an irrelevant antibody or an anti-human MMP14 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.WB
Western blot analysis was performed using anti-MMP14 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human MMP14 Lane 2:negative controlFC
Flow-cytometry using anti-human MMP14 antibody. BXPC3 cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human MMP14 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human MMP14 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1,k |
| Clone Number | Y8H1 |
| Host Species | Mouse |
| Tested Applications | BlockingELISAICCIFFCMWB |
| ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|
| Species Reactivity | Homo sapiens (Human) |
| Predicted Reactivity | Homo sapiens (Human) |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | MT-MMP 1, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MMP14, MTMMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP |
|---|---|
| Function | Anti-Human MMP14 Antibody (LEM-2/15)[LEM-2/15] (HW299013) is amouse monoclonalantibody detectingMT-MMP 1inBlocking, ELISA, ICC, IF, FCM, WB. Suitable forHuman.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | P50281 |
|---|
Bioactivity
Detects MMP14 in indirect ELISAs.
SDS-PAGE
SDS-PAGE for Anti-Human MMP14 Antibody .
FC
Flow-cytometry using anti-human MMP14 antibody.MDA-MB-231 cells were stained with an irrelevant antibody or an anti-human MMP14 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
WB
Western blot analysis was performed using anti-MMP14 monoclonal antibody at 1ug/mL on various samples.Lane 1:recombinant human MMP14 Lane 2:negative control
FC
Flow-cytometry using anti-human MMP14 antibody. BXPC3 cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human MMP14 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
