SDS-PAGE
SDS-PAGE for Anti-Mouse CD274/PD-L1/B7-H1 Antibody .FC
Flow-cytometry using APC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody or an APC anti-human CD274 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody or an FITC anti-human CD274 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using PE anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody or an PE anti-human CD274 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Mouse CD274/PD-L1/B7-H1 Recombinant Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,lambda |
| Clone Number | Z5G5 |
| Host Species | Mouse |
| Tested Applications | BlockingELISAFCM |
| ELISA:1:2000-1:20000 |
|
| Species Reactivity | Mouse |
| Predicted Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274 |
|---|---|
| Function | Anti-Mouse CD274/PD-L1/B7-H1 Antibody (SAA0142)[SAA0142] (MV974013) is amouse monoclonalantibody detectingB7-H1inBlocking, ELISA, FCM. Suitable forMouse.HighlightsMulti-Application— Validated across multiple applications.Flow Cytometry— Validated for flow cytometry applications. |
| SwissProt ID | Q9EP73 |
|---|
SDS-PAGE
SDS-PAGE for Anti-Mouse CD274/PD-L1/B7-H1 Antibody .
FC
Flow-cytometry using APC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody or an APC anti-human CD274 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using FITC anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody or an FITC anti-human CD274 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using PE anti-human CD274 antibody. MDA-MB-231 cells were stained with an irrelevant antibody or an PE anti-human CD274 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:2000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
