FC
Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were stained with an irrelevant antibody or an anti-human FOXP3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human FOXP3 antibody. Hela cells were stained with an irrelevant antibody or an anti-human FOXP3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human FOXP3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.FC
Flow-cytometry using anti-human FOXP3 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human FOXP3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Product Name | Anti-Human FOXP3 Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human FOXP3 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | F6Z9 |
| Host Species | Mouse |
| Tested Applications | ELISAFCMWB |
| ELISA:1:1000-1:10000 WB:1:200-1:2000 |
|
| Species Reactivity | Human |
| Predicted Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Target | Forkhead box protein P3, Scurfin, Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, FOXP3, IPEX, JM2 |
|---|---|
| Function | Anti-Human FOXP3 Monoclonal Antibody (1A209)[1A209] (HV388025) is amouse monoclonalantibody detectingFOXP3inELISA, FCM, WB. Suitable forHuman.Highlights●Recombinant— Superior batch-to-batch reproducibility with defined sequences.●Multi-Application— Validated across multiple applications.●Flow Cytometry— Validated for flow cytometry applications. |
FC
Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were stained with an irrelevant antibody or an anti-human FOXP3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human FOXP3 antibody. Hela cells were stained with an irrelevant antibody or an anti-human FOXP3 antibody monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human FOXP3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.
FC
Flow-cytometry using anti-human FOXP3 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody or an anti-human FOXP3 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.| Application Notes | ELISA:1:1000-1:10000 WB:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Storage Buffer | 0.01M PBS, pH 7.4.Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
