WB
Western blot analysis of AKT1/2/3 using anti-AKT1/2/3 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT1/2/3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for AKT1/2/3 at approximately 62 kDa. The expected band size for AKT1/2/3 is at 56 kDa.| Product Name | Anti-Zebrafish AKT1/2/3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish AKT1/2/3 recombinant protein (Position: E17-A474). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | AKT serine/threonine kinase 1,2,3 |
|---|---|
| Target | AKT1/AKT2/AKT3 |
| Alternative Names | AKT AKT1 PKB PKB ALPHA PRKBA Protein kinase B Proto oncogene c Akt RAC RAC ALPHA RAC PK alpha AKT2 PKB beta PKBB PKBBETA PRKBB Protein kinase Akt 2 Protein kinase B beta RAC BETA RAC PK beta AKT3 PKB GAMMA PKBG PRKBG Protein kinase Akt 3 Protein kinase B gamma RAC gamma RAC PK gamma STK 2 |
| Molecular Weight(MW) | 56 kDa(Observed band size: 62 kDa) |
| SwissProt ID | Q802Y3 |
|---|
WB
Western blot analysis of AKT1/2/3 using anti-AKT1/2/3 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT1/2/3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for AKT1/2/3 at approximately 62 kDa. The expected band size for AKT1/2/3 is at 56 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
