WB
Western blot analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha Tubulin/TUBA1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for Alpha Tubulin/TUBA1A at approximately 50 kDa. The expected band size for Alpha Tubulin/TUBA1A is at 50 kDa.
IHC
IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody .Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-Alpha Tubulin/TUBA1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody .Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-Alpha Tubulin/TUBA1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF
IF analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody .Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-Alpha Tubulin/TUBA1A Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody. The section was counterstained with DAPI .