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Anti-Zebrafish Alpha Tubulin/TUBA1A Antibody

Catalog Number:OM699001
Zebrafish
WB;IHC;IF
OM699001
Discount Price
OM699001-100μl
100μl
Product Profile
Product Name Anti-Zebrafish Alpha Tubulin/TUBA1A Antibody
Antibody Type Primary Antibodies
Immunogen E.coli-derived Zebrafish Alpha Tubulin/TUBA1A recombinant protein (Position: M1-G436).
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WBIHCIF
WB:1:500-1:2000
IHC:1:50-1:400
IF:1:50-1:400
Species Reactivity Zebrafish
Concentration 500ug/ml
Purification Affinity purified
Target Information
Gene Full Name tubulin, alpha 1a
Target TUBA1A
Alternative Names alpha tubulin
TUBA1A
TUBA3
Molecular Weight(MW) 50 kDa
Database Links
SwissProt ID Q6NWK7
Application

WB

Western blot analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha Tubulin/TUBA1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for Alpha Tubulin/TUBA1A at approximately 50 kDa. The expected band size for Alpha Tubulin/TUBA1A is at 50 kDa.

IHC

IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody .Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-Alpha Tubulin/TUBA1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody .Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-Alpha Tubulin/TUBA1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IF

IF analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody .Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-Alpha Tubulin/TUBA1A Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody. The section was counterstained with DAPI .
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
IF:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt,-20℃ as supplied.
Storage Buffer PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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