IF
IF analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody .ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .WB
Western blot analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: female zebrafish viscera tissue lysates,Lane 3: male zebrafish viscera tissue lysates,Lane 4: whole zebrafish tissue lysates,Lane 5: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATPB/ATP5F1B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for ATPB/ATP5F1B at approximately 50 kDa. The expected band size for ATPB/ATP5F1B is at 57 kDa.IHC
IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody .ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody .ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody .ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-Zebrafish ATPB/ATP5F1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish ATPB/ATP5F1B recombinant protein (Position: Q112-S517). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | ATP synthase F1 subunit beta |
|---|---|
| Target | ATP5F1B |
| Alternative Names | ATP5B ATPB ATPMB ATPSB Cell and organelle markers Mitochondria Marker Mitochondrial Marker Mitochondrion Marker |
| Molecular Weight(MW) | 57 kDa(Observed band size: 50 kDa) |
| SwissProt ID | A8WGC6 |
|---|
IF
IF analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody .ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .
WB
Western blot analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: female zebrafish viscera tissue lysates,Lane 3: male zebrafish viscera tissue lysates,Lane 4: whole zebrafish tissue lysates,Lane 5: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATPB/ATP5F1B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for ATPB/ATP5F1B at approximately 50 kDa. The expected band size for ATPB/ATP5F1B is at 57 kDa.
IHC
IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody .ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody .ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody .ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
