WB
Western blot analysis of Cavin1a using anti-Cavin1a antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: Zebrafish,Lane 2: Zebrafish head tissue lysates,Lane 3: Zebrafish body tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cavin1a antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for Cavin1a at approximately 47 kDa. The expected band size for Cavin1a is at 47 kDa.
IHC
IHC analysis of Cavin1a using anti-Cavin1a antibody .Cavin1a was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. The tissue section was incubated with rabbit anti-Cavin1a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Cavin1a using anti-Cavin1a antibody .Cavin1a was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-Cavin1a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Cavin1a using anti-Cavin1a antibody .Cavin1a was detected in a paraffin-embedded section of zebrafish muscle tissue. The tissue section was incubated with rabbit anti-Cavin1a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.