IF
IF analysis of cdhr1a using anti-cdhr1a antibody .cdhr1a was detected in a paraffin-embedded section of IF analysis of Cdhr1a using anti-Cdhr1a antibody .Cdhr1a was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Cdhr1a Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Fluoro488 Conjugated Avidin . Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-cdhr1a Antibody at a dilution of 1:100. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Fluoro 488 Conjugated Avidin .
IHC
IHC analysis of cdhr1a using anti-cdhr1a antibody .cdhr1a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-cdhr1a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of cdhr1a using anti-cdhr1a antibody .cdhr1a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-cdhr1a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.