WB
Western blot analysis of COL1A1A using anti-COL1A1A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COL1A1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for COL1A1A at approximately 137 kDa. The expected band size for COL1A1A is at 137 kDa.| Product Name | Anti-Zebrafish COL1A1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish COL1A1A recombinant protein (Position: F1180-M1341). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | collagen, type I, alpha 1a |
|---|---|
| Target | COL1A1A |
| Molecular Weight(MW) | 137 kDa |
| SwissProt ID | Q6U1J5 |
|---|
WB
Western blot analysis of COL1A1A using anti-COL1A1A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COL1A1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for COL1A1A at approximately 137 kDa. The expected band size for COL1A1A is at 137 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
