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Anti-Zebrafish DAPK2A Antibody

Catalog Number:OM699088
Zebrafish
WB;IHC
OM699088
Discount Price
OM699088-100μl
100μl
Product Profile
Product Name Anti-Zebrafish DAPK2A Antibody
Antibody Type Primary Antibodies
Immunogen E.coli-derived Zebrafish DAPK2A recombinant protein (Position: R263-R484).
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WBIHC
WB:1:500-1:2000
IHC:1:50-1:400
Species Reactivity Zebrafish
Concentration 500ug/ml
Purification Affinity purified
Target Information
Gene Full Name death-associated protein kinase 2a
Target DAPK2A
Molecular Weight(MW) 56 kDa
Database Links
SwissProt ID E7F1A1
Application

WB

Western blot analysis of DAPK2A using anti-DAPK2A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DAPK2A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for DAPK2A at approximately 56 kDa. The expected band size for DAPK2A is at 56 kDa.

IHC

IHC analysis of DAPK2A using anti-DAPK2A antibody .DAPK2A was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-DAPK2A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of DAPK2A using anti-DAPK2A antibody .DAPK2A was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-DAPK2A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt,-20℃ as supplied.
Storage Buffer PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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