WB
Western blot analysis of DPYD using anti-DPYD . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DPYD antigen affinity purified polyclonal at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for DPYD at approximately 111 kDa. The expected band size for DPYD is at 111 kDa.
IHC
IHC analysis of DPYD using anti-DPYD .DPYD was detected in a paraffin-embedded section of zebrafish eye tissue. The tissue section was incubated with rabbit anti-DPYD at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.