WB
Western blot analysis of DTNBA using anti-DTNBA . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DTNBA antigen affinity purified polyclonal at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for DTNBA at approximately 66 kDa. The expected band size for DTNBA is at 66 kDa.
IHC
IHC analysis of DTNBA using anti-DTNBA .DTNBA was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-DTNBA at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.