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Anti-Zebrafish E-cadherin/CDH1 Antibody

Catalog Number:OM699120
Zebrafish
WB;IHC
OM699120
Discount Price
OM699120-100μl
100μl
Product Profile
Product Name Anti-Zebrafish E-cadherin/CDH1 Antibody
Antibody Type Primary Antibodies
Immunogen E.coli-derived zebrafish E-cadherin/CDH1 recombinant protein (Position: Y23-D864)
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WBIHC
WB:1:500-1:2000
IHC:1:50-1:400
Species Reactivity Zebrafish
Concentration 500ug/ml
Purification Affinity purified
Target Information
Target CDH1
Alternative Names Arc 1
Cadherin 1
CD324
CDH1
CDHE
E cadherin
ECAD
E-cad
E-cadherin
LCAM
UVO
Uvomorulin
Molecular Weight(MW) 120 kDa(Observed band size: 130 kDa)
Database Links
SwissProt ID Q90Z37
Application

WB

Western blot analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates.Lane 2: whole female zebrafish tissue lysates.Lane 3: whole male zebrafish tissue lysates.Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-E-cadherin/CDH1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for E-cadherin/CDH1 at approximately 130 kDa. The expected band size for E-cadherin/CDH1 is at 120 kDa.

IHC

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody .E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish inner ear tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody .E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody .E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish esophageal epithelium tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody .E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody .E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody .E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody .E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt,-20℃ as supplied.
Storage Buffer PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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