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Anti-Zebrafish ETSRP Antibody

Catalog Number:OM699136
Zebrafish
WB;IHC
OM699136
Discount Price
OM699136-100μl
100μl
Product Profile
Product Name Anti-Zebrafish ETSRP Antibody
Antibody Type Primary Antibodies
Immunogen E.coli-derived zebrafish ETSRP recombinant protein (Position: M1-I218)
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WBIHC
WB:1:500-1:2000
IHC:1:50-1:400
Species Reactivity Zebrafish
Concentration 500ug/ml
Purification Affinity purified
Target Information
Target ETSRP
Molecular Weight(MW) 39 kDa
Database Links
SwissProt ID A0A2R8Q5A3
Application

WB

Western blot analysis of ETSRP using anti-ETSRP antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates.Lane 2: whole female zebrafish tissue lysates.Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ETSRP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for ETSRP at approximately 39 kDa. The expected band size for ETSRP is at 39 kDa.

IHC

IHC analysis of ETSRP using anti-ETSRP antibody .ETSRP was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-ETSRP Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt,-20℃ as supplied.
Storage Buffer PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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