IF
IF analysis of GBF1 using anti-GBF1 antibody .GBF1 was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-GBF1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .WB
Western blot analysis of GBF1 using anti-GBF1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GBF1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for GBF1 at approximately 207 kDa. The expected band size for GBF1 is at 207 kDa.IHC
IHC analysis of GBF1 using anti-GBF1 antibody .GBF1 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-GBF1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of GBF1 using anti-GBF1 antibody .GBF1 was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-GBF1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of GBF1 using anti-GBF1 antibody .GBF1 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-GBF1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-Zebrafish GBF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish GBF1 recombinant protein (Position: E110-K1605). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | GBF1 |
|---|---|
| Alternative Names | GBF1 KIAA0248 Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 BFA-resistant GEF 1 |
| Molecular Weight(MW) | 207 kDa |
| SwissProt ID | A0A286Y9X1 |
|---|
IF
IF analysis of GBF1 using anti-GBF1 antibody .GBF1 was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-GBF1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .
WB
Western blot analysis of GBF1 using anti-GBF1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GBF1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for GBF1 at approximately 207 kDa. The expected band size for GBF1 is at 207 kDa.
IHC
IHC analysis of GBF1 using anti-GBF1 antibody .GBF1 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-GBF1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of GBF1 using anti-GBF1 antibody .GBF1 was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-GBF1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of GBF1 using anti-GBF1 antibody .GBF1 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-GBF1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
