WB
Western blot analysis of GLSa/b using anti-GLSa/b antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLSa/b antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for GLSa/b at approximately 65 kDa. The expected band size for GLSa/b is at 73 kDa.IHC
IHC analysis of GLSa/b using anti-GLSa/b antibody .GLSa/b was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-GLSa/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of GLSa/b using anti-GLSa/b antibody .GLSa/b was detected in a paraffin-embedded section of zebrafish bile duct tissue. The tissue section was incubated with rabbit anti-GLSa/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of GLSa/b using anti-GLSa/b antibody .GLSa/b was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-GLSa/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-Zebrafish GLSa/b Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish GLSa/b recombinant protein (Position: K53-S312). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | GLSa/b |
|---|---|
| Alternative Names | AAD20 GAC GLS GLS1 glutaminase K glutaminase KGA KGA/GAC KIAA0838 L glutamine amidohydrolase |
| Molecular Weight(MW) | 73 kDa(Observed band size: 65 kDa) |
| SwissProt ID | A0A8M9PRT8 |
|---|
WB
Western blot analysis of GLSa/b using anti-GLSa/b antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLSa/b antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for GLSa/b at approximately 65 kDa. The expected band size for GLSa/b is at 73 kDa.
IHC
IHC analysis of GLSa/b using anti-GLSa/b antibody .GLSa/b was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-GLSa/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of GLSa/b using anti-GLSa/b antibody .GLSa/b was detected in a paraffin-embedded section of zebrafish bile duct tissue. The tissue section was incubated with rabbit anti-GLSa/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of GLSa/b using anti-GLSa/b antibody .GLSa/b was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-GLSa/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
