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Anti-Zebrafish HMOX1A Antibody
Catalog Number:OM699209
Zebrafish
WB;IHC
OM699209
Discount Price
OM699209-100μl
100μl
Product Profile
Product Name Anti-Zebrafish HMOX1A Antibody
Antibody Type Primary Antibodies
Immunogen E.coli-derived Zebrafish HMOX1A recombinant protein (Position: M1-K244)
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WBIHC
WB:1:500-1:2000
IHC:1:50-1:400
Species Reactivity Zebrafish
Concentration 500ug/ml
Purification Affinity purified
Target Information
Gene Full Name heme oxygenase 1a
Target HMOX1A
Alternative Names hmox1a
fc27c04
hmox1
Molecular Weight(MW) 30 kDa
Database Links
SwissProt ID B0UXS0
Application

WB

Western blot analysis of HMOX1A using anti-HMOX1A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HMOX1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for HMOX1A at approximately 30 kDa. The expected band size for HMOX1A is at 30 kDa.

IHC

IHC analysis of HMOX1A using anti-HMOX1A antibody .HMOX1A was detected in a paraffin-embedded section of zebrafish liver tissue. The tissue section was incubated with rabbit anti-HMOX1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of HMOX1A using anti-HMOX1A antibody .HMOX1A was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-HMOX1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt,-20℃ as supplied.
Storage Buffer PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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