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Anti-Zebrafish HNRNPD Antibody

Catalog Number:OM699212
Zebrafish
IF;WB;IHC
OM699212
Discount Price
OM699212-100μl
100μl
Product Profile
Product Name Anti-Zebrafish HNRNPD Antibody
Antibody Type Primary Antibodies
Immunogen E.coli-derived zebrafish HNRNPD recombinant protein (Position: K43-N203)
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications IFWBIHC
WB:1:500-1:2000
IHC:1:50-1:400
IF:1:50-1:400
Species Reactivity Zebrafish
Concentration 500ug/ml
Purification Affinity purified
Target Information
Target HNRNPD
Alternative Names AUF1
AUF1A
hnRNP D0
HNRNPD
hnRNPD0
HNRPD
P37
Molecular Weight(MW) 38 kDa(Observed band size: 40 kDa)
Database Links
SwissProt ID A0A8M9QC46
Application

IF

IF analysis of HNRNPD using anti-HNRNPD antibody .HNRNPD was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-HNRNPD Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.

WB

Western blot analysis of HNRNPD using anti-HNRNPD antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HNRNPD antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for HNRNPD at approximately 40 kDa. The expected band size for HNRNPD is at 38 kDa.

IHC

IHC analysis of HNRNPD using anti-HNRNPD antibody .HNRNPD was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-HNRNPD Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of HNRNPD using anti-HNRNPD antibody .HNRNPD was detected in a paraffin-embedded section of zebrafish cerebellum tissue. The tissue section was incubated with rabbit anti-HNRNPD Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of HNRNPD using anti-HNRNPD antibody .HNRNPD was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-HNRNPD Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of HNRNPD using anti-HNRNPD antibody .HNRNPD was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-HNRNPD Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of HNRNPD using anti-HNRNPD antibody .HNRNPD was detected in a paraffin-embedded section of zebrafish muscle tissue. The tissue section was incubated with rabbit anti-HNRNPD Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
IF:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt,-20℃ as supplied.
Storage Buffer PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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