WB
Western blot analysis of HSP90A using anti-HSP90A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSP90A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for HSP90A at approximately 95 kDa. The expected band size for HSP90A is at 83 kDa.IHC
IHC analysis of HSP90A using anti-HSP90A antibody .HSP90A was detected in a paraffin-embedded section of zebrafish muscle tissue. The tissue section was incubated with rabbit anti-HSP90A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HSP90A using anti-HSP90A antibody .HSP90A was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-HSP90A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HSP90A using anti-HSP90A antibody .HSP90A was detected in a paraffin-embedded section of zebrafish cardiac muscle tissue. The tissue section was incubated with rabbit anti-HSP90A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF
IF analysis of HSP90A using anti-HSP90A antibody .HSP90A was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-HSP90A Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody. The section was counterstained with DAPI .| Product Name | Anti-Zebrafish HSP90A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish HSP90A recombinant protein (Position: R393-G688). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | heat shock protein 90, alpha (cytosolic), class A member 1, tandem duplicate 1 |
|---|---|
| Target | HSP90A |
| Alternative Names | hsp90 hsp90a hsp90aa1 fb17b01 hsp90a.1 zgc:86652 hsp90alpha wu:fb17b01 |
| Molecular Weight(MW) | 83 kDa(Observed band size: 95 kDa) |
| SwissProt ID | Q90474 |
|---|
WB
Western blot analysis of HSP90A using anti-HSP90A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSP90A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for HSP90A at approximately 95 kDa. The expected band size for HSP90A is at 83 kDa.
IHC
IHC analysis of HSP90A using anti-HSP90A antibody .HSP90A was detected in a paraffin-embedded section of zebrafish muscle tissue. The tissue section was incubated with rabbit anti-HSP90A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HSP90A using anti-HSP90A antibody .HSP90A was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-HSP90A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HSP90A using anti-HSP90A antibody .HSP90A was detected in a paraffin-embedded section of zebrafish cardiac muscle tissue. The tissue section was incubated with rabbit anti-HSP90A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF
IF analysis of HSP90A using anti-HSP90A antibody .HSP90A was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-HSP90A Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody. The section was counterstained with DAPI .| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
