WB
Western blot analysis of HSPA5 using anti-HSPA5 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSPA5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for HSPA5 at approximately 80 kDa. The expected band size for HSPA5 is at 72 kDa.IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish liver tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish pancreas tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish testis tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-Zebrafish HSPA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish HSPA5 recombinant protein (Position: D103-L650) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | heat shock protein 5 |
|---|---|
| Target | HSPA5 |
| Alternative Names | BIP GRP 78 GRP78 GRP78/BIP HSP A5 HSPA5 MIF2 |
| Molecular Weight(MW) | 72 kDa(Observed band size: 80 kDa) |
| SwissProt ID | Q7SZD3 |
|---|
WB
Western blot analysis of HSPA5 using anti-HSPA5 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSPA5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for HSPA5 at approximately 80 kDa. The expected band size for HSPA5 is at 72 kDa.
IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish liver tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish pancreas tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish testis tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HSPA5 using anti-HSPA5 antibody .HSPA5 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-HSPA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
