WB
Western blot analysis of LIN7C using anti-LIN7C antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LIN7C antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for LIN7C at approximately 25 kDa. The expected band size for LIN7C is at 22 kDa.| Product Name | Anti-Zebrafish LIN7C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish LIN7C recombinant protein (Position: M1-A73) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | LIN7C |
|---|---|
| Alternative Names | LIN 7 C lin 7 homolog C (C. elegans) LIN 7C LIN7C MALS 3 MALS3 Mammalian lin seven protein 3 Protein lin 7 homolog C Veli 3 VELI3 Vertebrate lin 7 homolog 3 |
| Molecular Weight(MW) | 22 kDa(Observed band size: 25 kDa) |
| SwissProt ID | Q66IB0 |
|---|
WB
Western blot analysis of LIN7C using anti-LIN7C antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LIN7C antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for LIN7C at approximately 25 kDa. The expected band size for LIN7C is at 22 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
