WB
Western blot analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates.Lane 2: whole female zebrafish tissue lysates.Lane 3: whole male zebrafish tissue lysates.Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lysozyme/LYZ antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for Lysozyme/LYZ at approximately 15 kDa. The expected band size for Lysozyme/LYZ is at 17 kDa.
IHC
IHC analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody .Lysozyme/LYZ was detected in a paraffin-embedded section of zebrafish liver tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody .Lysozyme/LYZ was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.