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Anti-Zebrafish MAT2Aa/b Antibody

Catalog Number:OM699284
Zebrafish
WB;IHC
OM699284
Discount Price
OM699284-100μl
100μl
Product Profile
Product Name Anti-Zebrafish MAT2Aa/b Antibody
Antibody Type Primary Antibodies
Immunogen E.coli-derived zebrafish MAT2Aa/b recombinant protein (Position: M1-A229)
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WBIHC
WB:1:500-1:2000
IHC:1:50-1:400
Species Reactivity Zebrafish
Concentration 500ug/ml
Purification Affinity purified
Target Information
Target MAT2Aa/b
Alternative Names AdoMet synthase 2
AMS2
MAT 2
MAT II
MAT2A
MATA2
MATII
SAMS2
Molecular Weight(MW) 44 kDa(Observed band size: 60 kDa)
Database Links
SwissProt ID F1QYU7A0A8M6YZ65
Application

WB

Western blot analysis of MAT2Aa/b using anti-MAT2Aa/b antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAT2Aa/b antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for MAT2Aa/b at approximately 60 kDa. The expected band size for MAT2Aa/b is at 44 kDa.

IHC

IHC analysis of MAT2Aa/b using anti-MAT2Aa/b antibody .MAT2Aa/b was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-MAT2Aa/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC

IHC analysis of MAT2Aa/b using anti-MAT2Aa/b antibody .MAT2Aa/b was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-MAT2Aa/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt,-20℃ as supplied.
Storage Buffer PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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