WB
Western blot analysis of METAP2a/b using anti-METAP2a/b antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-METAP2a/b antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for METAP2a/b at approximately 67 kDa. The expected band size for METAP2a/b is at 53 kDa.IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish muscle tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish liver tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-Zebrafish METAP2a/b Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish METAP2a/b recombinant protein (Position: E191-R464) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | METAP2a/b |
|---|---|
| Alternative Names | MAP 2 MAP2 MetAP 2 METAP2 Methionine aminopeptidase 2 methionyl aminopeptidase 2 MNPEP p67 p67eIF2 Peptidase M 2 |
| Molecular Weight(MW) | 53 kDa(Observed band size: 67 kDa) |
| SwissProt ID | A5WVX8Q7SXK1 |
|---|
WB
Western blot analysis of METAP2a/b using anti-METAP2a/b antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-METAP2a/b antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for METAP2a/b at approximately 67 kDa. The expected band size for METAP2a/b is at 53 kDa.
IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish heart tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish muscle tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish liver tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of METAP2a/b using anti-METAP2a/b antibody .METAP2a/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. The tissue section was incubated with rabbit anti-METAP2a/b Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
