WB
Western blot analysis of MKP-1/DUSP1 using anti-MKP-1/DUSP1 . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MKP-1/DUSP1 antigen affinity purified polyclonal at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for MKP-1/DUSP1 at approximately 40 kDa. The expected band size for MKP-1/DUSP1 is at 40 kDa.| Product Name | Anti-Zebrafish MKP-1/DUSP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish MKP-1/DUSP1 recombinant protein (Position: M1-P353). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | dual specificity phosphatase 1 |
|---|---|
| Target | DUSP1 |
| Alternative Names | Dual specificity phosphatase 1 CL100 HVH1 MKP-1 MKP1 PTPN10 DUSP1 MKP-1/2 |
| Molecular Weight(MW) | 40 kDa |
| SwissProt ID | Q7ZVL8 |
|---|
WB
Western blot analysis of MKP-1/DUSP1 using anti-MKP-1/DUSP1 . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MKP-1/DUSP1 antigen affinity purified polyclonal at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for MKP-1/DUSP1 at approximately 40 kDa. The expected band size for MKP-1/DUSP1 is at 40 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
