WB
Western blot analysis of MRC1A using anti-MRC1A . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MRC1A antigen affinity purified polyclonal at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for MRC1A at approximately 165 kDa. The expected band size for MRC1A is at 163 kDa.