WB
Western blot analysis of MYO6A using anti-MYO6A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MYO6A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for MYO6A at approximately 150 kDa. The expected band size for MYO6A is at 144 kDa.
IHC
IHC analysis of MYO6A using anti-MYO6A antibody .MYO6A was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-MYO6A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.