WB
Western blot analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NF-κB p65/RELA antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for NF-κB p65/RELA at approximately 70 kDa. The expected band size for NF-κB p65/RELA is at 65 kDa.| Product Name | Anti-Zebrafish NF-κB p65/RELA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish NF-κB p65/RELA recombinant protein (Position: M1-F588). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | Enables DNA-binding transcription factor activity, RNA polymerase II-specific. Acts upstream of or within several processes, including defense response to virus; positive regulation of DNA-templated transcription; and regulation of apoptotic process. Located in nucleus. Is expressed in intestine; kidney; liver; male organism; and posterior intestine. Human ortholog(s) of this gene implicated in ductal carcinoma in situ; lung non-small cell carcinoma; lymphoma; and renal cell carcinoma. Orthologous to human RELA (RELA proto-oncogene, NF-kB subunit). |
|---|---|
| Target | RELA |
| Alternative Names | NF Kappa B NF κB NFKB 3 NFKB p65 NFKB3 NF-κB NF-κB3 p65 RELA Transcription factor p65 |
| Molecular Weight(MW) | 65 kDa(Observed band size: 70 kDa) |
| SwissProt ID | A0A8M2BI19 |
|---|
WB
Western blot analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NF-κB p65/RELA antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for NF-κB p65/RELA at approximately 70 kDa. The expected band size for NF-κB p65/RELA is at 65 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
