IHC
IHC analysis of NFKB1 using anti-NFKB1 antibody .NFKB1 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-NFKB1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.WB
Western blot analysis of NFKB1 using anti-NFKB1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFKB1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for NFKB1 at approximately 55 kDa. The expected band size for NFKB1 is at 50 kDa.| Product Name | Anti-Zebrafish NFKB1 p105/NFKB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish NFKB1 recombinant protein (Position: E10-K364). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 |
|---|---|
| Target | NFKB1 |
| Alternative Names | NFKB1 DNA binding factor KBF1 DNA-binding factor KBF1 EBP 1 EBP-1 |
| Molecular Weight(MW) | 50 kDa(Observed band size: 55 kDa) |
| SwissProt ID | A0A8M9QGX8 |
|---|
IHC
IHC analysis of NFKB1 using anti-NFKB1 antibody .NFKB1 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-NFKB1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
WB
Western blot analysis of NFKB1 using anti-NFKB1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFKB1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for NFKB1 at approximately 55 kDa. The expected band size for NFKB1 is at 50 kDa.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
