IF
IF analysis of nlz2 znf503 using anti-nlz2 znf503 antibody .nlz2 znf503 was detected in a paraffin-embedded section of IF analysis of nlz2 using anti-nlz2 antibody .nlz2 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti-nlz2 Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-nlz2 znf503 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.WB
Western blot analysis of nlz2 znf503 using anti-nlz2 znf503 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: Marker,Lane 2: Zebrafish tissue lysates,Lane 3: Zebrafish head tissue lysates,Lane 4: Zebrafish body tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-nlz2 znf503 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for nlz2 znf503 at approximately 63 kDa. The expected band size for nlz2 znf503 is at 63 kDa.| Product Name | Anti-Zebrafish nlz2 znf503 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| WB:1:500-1:2000 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | znf503 |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| SwissProt ID | Q6UFS5 |
|---|
IF
IF analysis of nlz2 znf503 using anti-nlz2 znf503 antibody .nlz2 znf503 was detected in a paraffin-embedded section of IF analysis of nlz2 using anti-nlz2 antibody .nlz2 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti-nlz2 Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-nlz2 znf503 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.
WB
Western blot analysis of nlz2 znf503 using anti-nlz2 znf503 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: Marker,Lane 2: Zebrafish tissue lysates,Lane 3: Zebrafish head tissue lysates,Lane 4: Zebrafish body tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-nlz2 znf503 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for nlz2 znf503 at approximately 63 kDa. The expected band size for nlz2 znf503 is at 63 kDa.| Application Notes | WB:1:500-1:2000 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
