WB
Western blot analysis of PKLR using anti-PKLR antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: whole female zebrafish tissue lysates,Lane 2: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKLR antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for PKLR at approximately 57 kDa. The expected band size for PKLR is at 57 kDa.| Product Name | Anti-Zebrafish PKLR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish PKLR recombinant protein (Position: D31-D503). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | pyruvate kinase L/R |
|---|---|
| Target | PKLR |
| Alternative Names | EC:2.7.1.40 PK1 PKL Pyruvate kinase isozymes L/R Pyruvate kinase PKLR |
| Molecular Weight(MW) | 57 kDa |
| SwissProt ID | A0A8M9NZ14 |
|---|
WB
Western blot analysis of PKLR using anti-PKLR antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: whole female zebrafish tissue lysates,Lane 2: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKLR antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for PKLR at approximately 57 kDa. The expected band size for PKLR is at 57 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
