WB
Western blot analysis of POLR2B using anti-POLR2B antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLR2B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for POLR2B at approximately 130 kDa. The expected band size for POLR2B is at 134 kDa.| Product Name | Anti-Zebrafish POLR2B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish POLR2B recombinant protein (Position: E49-Q1145). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | POLR2B |
|---|---|
| Alternative Names | hRPB140 hsRPB2 POL2RB POLR2B POLR2B-Specific RNA polymerase II subunit 2 RNA polymerase II subunit B2 RPB2 |
| Molecular Weight(MW) | 134 kDa(Observed band size: 130 kDa) |
| SwissProt ID | A0A8M1N6S0 |
|---|
WB
Western blot analysis of POLR2B using anti-POLR2B antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLR2B antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for POLR2B at approximately 130 kDa. The expected band size for POLR2B is at 134 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
