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Anti-Zebrafish PROX1a Antibody

Catalog Number:OM699385
Zebrafish
WB;IHC
OM699385
Discount Price
OM699385-100μl
100μl
Product Profile
Product Name Anti-Zebrafish PROX1a Antibody
Antibody Type Primary Antibodies
Immunogen E.coli-derived zebrafish PROX1a recombinant protein (Position: E51-E739).
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WBIHC
WB:1:500-1:2000
IHC:1:50-1:400
Species Reactivity Zebrafish
Concentration 500ug/ml
Purification Affinity purified
Target Information
Target PROX1a
Alternative Names prospero homeobox 1
Prospero homeobox protein 1
PROX 1
PROX1
Molecular Weight(MW) 83 kDa(Observed band size: 95 kDa)
Database Links
SwissProt ID F1QAE1
Application

WB

Western blot analysis of PROX1a using anti-PROX1a antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PROX1a antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for PROX1a at approximately 95 kDa. The expected band size for PROX1a is at 83 kDa.

IHC

IHC analysis of PROX1a using anti-PROX1a antibody .PROX1a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-PROX1a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt,-20℃ as supplied.
Storage Buffer PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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