WB
Western blot analysis of PROX1a using anti-PROX1a antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PROX1a antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for PROX1a at approximately 95 kDa. The expected band size for PROX1a is at 83 kDa.
IHC
IHC analysis of PROX1a using anti-PROX1a antibody .PROX1a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-PROX1a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.