IF
IF analysis of PRPF6 using anti-PRPF6 antibody .PRPF6 was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-PRPF6 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody.WB
Western blot analysis of PRPF6 using anti-PRPF6 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRPF6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for PRPF6 at approximately 107 kDa. The expected band size for PRPF6 is at 107 kDa.| Product Name | Anti-Zebrafish PRPF6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish PRPF6 recombinant protein (Position: Q99-K618). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| WB:1:500-1:2000 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | PRPF6 |
|---|---|
| Alternative Names | ANT 1 C20orf14 hPrp6 Pre mRNA processing factor 6 PRP6 homolog PRPF6 TOM U5 102 kDa protein U5 102K |
| Molecular Weight(MW) | 107 kDa |
| SwissProt ID | A0A2R8PWG5 |
|---|
IF
IF analysis of PRPF6 using anti-PRPF6 antibody .PRPF6 was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-PRPF6 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody.
WB
Western blot analysis of PRPF6 using anti-PRPF6 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRPF6 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for PRPF6 at approximately 107 kDa. The expected band size for PRPF6 is at 107 kDa.| Application Notes | WB:1:500-1:2000 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
