WB
Western blot analysis of PSMA5 using anti-PSMA5 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSMA5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for PSMA5 at approximately 26 kDa. The expected band size for PSMA5 is at 26 kDa.
IHC
IHC analysis of PSMA5 using anti-PSMA5 antibody .PSMA5 was detected in a paraffin-embedded section of zebrafish spinal tissue. The tissue section was incubated with rabbit anti-PSMA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PSMA5 using anti-PSMA5 antibody .PSMA5 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-PSMA5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.