WB
Western blot analysis of PSMC4 using anti-PSMC4 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSMC4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for PSMC4 at approximately 47 kDa. The expected band size for PSMC4 is at 47 kDa.
IHC
IHC analysis of PSMC4 using anti-PSMC4 antibody .PSMC4 was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-PSMC4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.