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Anti-Zebrafish PSMC4 Antibody

Catalog Number:OM699395
Zebrafish
WB;IHC
OM699395
Discount Price
OM699395-100μl
100μl
Product Profile
Product Name Anti-Zebrafish PSMC4 Antibody
Antibody Type Primary Antibodies
Immunogen E.coli-derived zebrafish PSMC4 recombinant protein (Position: M1-D410)
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WBIHC
WB:1:500-1:2000
IHC:1:50-1:400
Species Reactivity Zebrafish
Concentration 500ug/ml
Purification Affinity purified
Target Information
Target PSMC4
Alternative Names MB67 interacting protein
MIP224
PSMC4
S6
Tat binding protein 7
TBP 7
TBP7
Molecular Weight(MW) 47 kDa
Database Links
SwissProt ID B0R1D0
Application

WB

Western blot analysis of PSMC4 using anti-PSMC4 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSMC4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for PSMC4 at approximately 47 kDa. The expected band size for PSMC4 is at 47 kDa.

IHC

IHC analysis of PSMC4 using anti-PSMC4 antibody .PSMC4 was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-PSMC4 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Application Notes WB:1:500-1:2000
IHC:1:50-1:400
Additional Information
Form Liquid
Storage Instructions 12 months from date of receipt,-20℃ as supplied.
Storage Buffer PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
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