WB
Western blot analysis of RAB10 using anti-RAB10 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: whole female zebrafish tissue lysates,Lane 2: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB10 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for RAB10 at approximately 22 kDa. The expected band size for RAB10 is at 23 kDa.| Product Name | Anti-Zebrafish RAB10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish RAB10 recombinant protein (Position: A153-C200). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | RAB10, member RAS oncogene family |
|---|---|
| Target | RAB10 |
| Alternative Names | RAB10 Ras related protein Rab 10 |
| Molecular Weight(MW) | 23 kDa(Observed band size: 22 kDa) |
| SwissProt ID | Q6DGV5 |
|---|
WB
Western blot analysis of RAB10 using anti-RAB10 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: whole female zebrafish tissue lysates,Lane 2: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB10 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for RAB10 at approximately 22 kDa. The expected band size for RAB10 is at 23 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
