WB
Western blot analysis of SF3A3 using anti-SF3A3 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SF3A3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for SF3A3 at approximately 59 kDa. The expected band size for SF3A3 is at 59 kDa.| Product Name | Anti-Zebrafish SF3A3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish SF3A3 recombinant protein (Position: E123-Q472). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | splicing factor 3a, subunit 3 |
|---|---|
| Target | SF3A3 |
| Alternative Names | PRP9 PRPF9 SAP 61 SAP61 SF3A3 SF3a60 Splicing factor 3A subunit 3 |
| Molecular Weight(MW) | 59 kDa |
| SwissProt ID | Q6DRK2 |
|---|
WB
Western blot analysis of SF3A3 using anti-SF3A3 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SF3A3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for SF3A3 at approximately 59 kDa. The expected band size for SF3A3 is at 59 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
