IF
IF analysis of si:dkeyp-75b4.10/eslec using anti-si:dkeyp-75b4.10/eslec antibody .si:dkeyp-75b4.10/eslec was detected in a paraffin-embedded section of IF analysis of zebrafish eosinophils using Anti-Zebrafish Si:Dkeyp-75b4.10 Antibody .Si:Dkeyp-75b4.10 was detected in zebrafish eosinophils in FFPE tissue sections and fixed cells. Heat mediated antigen retrieval was performed in EDTA buffer . The tissue section was blocked with 3% goat serum in PBS. The tissue section was then incubated with 4 μg/mL rabbit anti-GFAP antibody and anti-MBP antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Mouse IgG , Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:1000 dilution and incubated for 1 hour at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-si:dkeyp-75b4.10/eslec Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody were used as secondary antibody. The section was counterstained with DAPI .WB
Western blot analysis of si:dkeyp-75b4.10/eslec using anti-si:dkeyp-75b4.10/eslec antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: Marker,Lane 2: Zebrafish,Lane 3: Zebrafish body tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-si:dkeyp-75b4.10/eslec antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for si:dkeyp-75b4.10/eslec at approximately 18 kDa. The expected band size for si:dkeyp-75b4.10/eslec is at 18 kDa.| Product Name | Anti-Zebrafish si:dkeyp-75b4.10/eslec Antibody |
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| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| WB:1:500-1:2000 IF:1:50-1:400 |
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| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 18 kDa |
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IF
IF analysis of si:dkeyp-75b4.10/eslec using anti-si:dkeyp-75b4.10/eslec antibody .si:dkeyp-75b4.10/eslec was detected in a paraffin-embedded section of IF analysis of zebrafish eosinophils using Anti-Zebrafish Si:Dkeyp-75b4.10 Antibody .Si:Dkeyp-75b4.10 was detected in zebrafish eosinophils in FFPE tissue sections and fixed cells. Heat mediated antigen retrieval was performed in EDTA buffer . The tissue section was blocked with 3% goat serum in PBS. The tissue section was then incubated with 4 μg/mL rabbit anti-GFAP antibody and anti-MBP antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Mouse IgG , Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:1000 dilution and incubated for 1 hour at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-si:dkeyp-75b4.10/eslec Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody were used as secondary antibody. The section was counterstained with DAPI .
WB
Western blot analysis of si:dkeyp-75b4.10/eslec using anti-si:dkeyp-75b4.10/eslec antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: Marker,Lane 2: Zebrafish,Lane 3: Zebrafish body tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-si:dkeyp-75b4.10/eslec antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for si:dkeyp-75b4.10/eslec at approximately 18 kDa. The expected band size for si:dkeyp-75b4.10/eslec is at 18 kDa.| Application Notes | WB:1:500-1:2000 IF:1:50-1:400 |
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| Form | Liquid |
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| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
