IF
IF analysis of siah1 using anti-siah1 antibody .siah1 was detected in a paraffin-embedded section of IF analysis of Siah1 using anti-Siah1 antibody .Siah1 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Siah1 Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Fluoro594 Conjugated Avidin . Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-siah1 Antibody at a dilution of 1:100. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Fluoro 594 Conjugated Avidin .| Product Name | Anti-Zebrafish siah1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | siah1 |
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| SwissProt ID | A0A0R4IPD0 |
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IF
IF analysis of siah1 using anti-siah1 antibody .siah1 was detected in a paraffin-embedded section of IF analysis of Siah1 using anti-Siah1 antibody .Siah1 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Siah1 Antibody overnight at 4°C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Fluoro594 Conjugated Avidin . Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-siah1 Antibody at a dilution of 1:100. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Fluoro 594 Conjugated Avidin .| Application Notes | IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
