IF
IF analysis of SMARCA4A using anti-SMARCA4A antibody .SMARCA4A was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-SMARCA4A Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody.WB
Western blot analysis of SMARCA4A using anti-SMARCA4A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMARCA4A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for SMARCA4A at approximately 220 kDa. The expected band size for SMARCA4A is at 185 kDa.IHC
IHC analysis of SMARCA4A using anti-SMARCA4A antibody .SMARCA4A was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-SMARCA4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SMARCA4A using anti-SMARCA4A antibody .SMARCA4A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-SMARCA4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SMARCA4A using anti-SMARCA4A antibody .SMARCA4A was detected in a paraffin-embedded section of zebrafish eye tissue. The tissue section was incubated with rabbit anti-SMARCA4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-Zebrafish SMARCA4A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived zebrafish SMARCA4A recombinant protein (Position: Q573-E775). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | SWI/SNF related BAF chromatin remodeling complex subunit ATPase 4a |
|---|---|
| Target | SMARCA4A |
| Alternative Names | ATP dependent helicase SMARCA4 BAF190 BAF190A BRG1 BRG1 associated factor 190A hSNF2b Protein brahma homolog 1 Protein BRG 1 SMARCA4 SMARCA4/BRG1 SNF2 SNF2 BETA SNF2B SNF2L4 SNF2LB SWI2 Transcription activator BRG1 |
| Molecular Weight(MW) | 185 kDa(Observed band size: 220 kDa) |
| SwissProt ID | Q7ZSY3 |
|---|
IF
IF analysis of SMARCA4A using anti-SMARCA4A antibody .SMARCA4A was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-SMARCA4A Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody.
WB
Western blot analysis of SMARCA4A using anti-SMARCA4A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMARCA4A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for SMARCA4A at approximately 220 kDa. The expected band size for SMARCA4A is at 185 kDa.
IHC
IHC analysis of SMARCA4A using anti-SMARCA4A antibody .SMARCA4A was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-SMARCA4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SMARCA4A using anti-SMARCA4A antibody .SMARCA4A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-SMARCA4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SMARCA4A using anti-SMARCA4A antibody .SMARCA4A was detected in a paraffin-embedded section of zebrafish eye tissue. The tissue section was incubated with rabbit anti-SMARCA4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
