WB
Western blot analysis of STAT3 using anti-STAT3 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for STAT3 at approximately 91 kDa. The expected band size for STAT3 is at 91 kDa.IHC
IHC analysis of STAT3 using anti-STAT3 antibody .STAT3 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-STAT3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF
IF analysis of STAT3 using anti-STAT3 antibody .STAT3 was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-STAT3 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.| Product Name | Anti-Zebrafish STAT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish STAT3 recombinant protein (Position: K64-S335) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | signal transducer and activator of transcription 3 (acute-phase response factor) |
|---|---|
| Target | STAT3 |
| Alternative Names | Acute phase response factor APRF HIES STAT3 |
| Molecular Weight(MW) | 91 kDa |
| SwissProt ID | A0A8M2BAX1 |
|---|
WB
Western blot analysis of STAT3 using anti-STAT3 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for STAT3 at approximately 91 kDa. The expected band size for STAT3 is at 91 kDa.
IHC
IHC analysis of STAT3 using anti-STAT3 antibody .STAT3 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-STAT3 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF
IF analysis of STAT3 using anti-STAT3 antibody .STAT3 was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-STAT3 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
