IF
IF analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of IF analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4 μg/mL rabbit anti-Tfap2a Antibody overnight at 4°C. Fluoro594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .IHC
IHC analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish esophagus tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-Zebrafish Tfap2a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| IHC:1:50-1:400 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | tfap2a |
|---|
| SwissProt ID | A0A2R8QRQ5 |
|---|
IF
IF analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of IF analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4 μg/mL rabbit anti-Tfap2a Antibody overnight at 4°C. Fluoro594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody was used as secondary antibody. The section was counterstained with DAPI .
IHC
IHC analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish esophagus tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of Tfap2a using anti-Tfap2a antibody .Tfap2a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-Tfap2a Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | IHC:1:50-1:400 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
