WB
Western blot analysis of TGFB1A using anti-TGFB1A antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGFB1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for TGFB1A at approximately 40 kDa. The expected band size for TGFB1A is at 43 kDa.
IHC
IHC analysis of TGFB1A using anti-TGFB1A antibody .TGFB1A was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-TGFB1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TGFB1A using anti-TGFB1A antibody .TGFB1A was detected in a paraffin-embedded section of zebrafish colon tissue. The tissue section was incubated with rabbit anti-TGFB1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TGFB1A using anti-TGFB1A antibody .TGFB1A was detected in a paraffin-embedded section of zebrafish liver tissue. The tissue section was incubated with rabbit anti-TGFB1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TGFB1A using anti-TGFB1A antibody .TGFB1A was detected in a paraffin-embedded section of zebrafish testis tissue. The tissue section was incubated with rabbit anti-TGFB1A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.