WB
Western blot analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TWIST1A/1B/2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for TWIST1A/1B/2 at approximately 28 kDa. The expected band size for TWIST1A/1B/2 is at 21 kDa.IHC
IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody .TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-TWIST1A/1B/2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody .TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-TWIST1A/1B/2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody .TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish muscle tissue. The tissue section was incubated with rabbit anti-TWIST1A/1B/2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody .TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-TWIST1A/1B/2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-Zebrafish TWIST1A/1B/2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of zebrafish TWIST1A/1B/2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | twist family bHLH transcription factor 1a |
|---|---|
| Target | TWIST1A/1B/2 |
| Alternative Names | TWIST1 BHLHA38 TWIST bHLHa38 H-twist TWIST2 BHLHA39 DERMO1 Twist-related protein 2 bHLHa39 Dermo-1 |
| Molecular Weight(MW) | 21 kDa(Observed band size: 28 kDa) |
| SwissProt ID | Q9PTE3Q568M7Q5XJI8 |
|---|
WB
Western blot analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TWIST1A/1B/2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for TWIST1A/1B/2 at approximately 28 kDa. The expected band size for TWIST1A/1B/2 is at 21 kDa.
IHC
IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody .TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-TWIST1A/1B/2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody .TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. The tissue section was incubated with rabbit anti-TWIST1A/1B/2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody .TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish muscle tissue. The tissue section was incubated with rabbit anti-TWIST1A/1B/2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody .TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-TWIST1A/1B/2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
