IF
IF analysis of Ush2a using anti-Ush2a antibody .Ush2a was detected in a paraffin-embedded section of IF analysis of Ush2a using anti-Ush2a antibody .Ush2a was detected in cryosections of wildtype zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody in blocking solution at 1:200 dilution overnight at 4°C. Alexa Fluor® 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold. tissue. The tissue section was incubated with rabbit anti-Ush2a Antibody at a dilution of 1:100. Alexa Fluor 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.| Product Name | Anti-Zebrafish Ush2a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Target | ush2a |
|---|
| SwissProt ID | Q1MTD2 |
|---|
IF
IF analysis of Ush2a using anti-Ush2a antibody .Ush2a was detected in a paraffin-embedded section of IF analysis of Ush2a using anti-Ush2a antibody .Ush2a was detected in cryosections of wildtype zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody in blocking solution at 1:200 dilution overnight at 4°C. Alexa Fluor® 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold. tissue. The tissue section was incubated with rabbit anti-Ush2a Antibody at a dilution of 1:100. Alexa Fluor 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody.| Application Notes | IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
