WB
Western blot analysis of wfikkn1 using anti-wfikkn1 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: Zebrafish,Lane 2: Zebrafish head tissue lysates,Lane 3: Zebrafish body tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-wfikkn1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for wfikkn1 at approximately 62 kDa. The expected band size for wfikkn1 is at 62 kDa.