WB
Western blot analysis of WNT8A using anti-WNT8A . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-WNT8A antigen affinity purified polyclonal at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for WNT8A at approximately 42 kDa. The expected band size for WNT8A is at 40 kDa.IHC
IHC analysis of WNT8A using anti-WNT8A .WNT8A was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-WNT8A at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-Zebrafish WNT8A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish WNT8A recombinant protein (Position: S134-R359). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | wingless-type MMTV integration site family, member 8a |
|---|---|
| Target | WNT8A |
| Alternative Names | Wnt family member 8A WNT8D WNT8A |
| Molecular Weight(MW) | 40 kDa(Observed band size: 42 kDa) |
| SwissProt ID | P51028 |
|---|
WB
Western blot analysis of WNT8A using anti-WNT8A . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-WNT8A antigen affinity purified polyclonal at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for WNT8A at approximately 42 kDa. The expected band size for WNT8A is at 40 kDa.
IHC
IHC analysis of WNT8A using anti-WNT8A .WNT8A was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-WNT8A at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
